Effect of Yunvjian with or Without Achyranthis Bidentatae Radix on Glucose and Lipid Metabolism and Inflammatory Response in Diabetic Rats with Syndrome of Yin Deficiency and Internal Heat / 中国实验方剂学杂志

ObjectiveTo investigate the different effects of Yunvjian with or without Achyranthis Bidentatae Radix on glucose and lipid metabolism and inflammatory response in diabetic rats with the syndrome of Yin deficiency and internal heat. MethodThe rat model of diabetes due to Yin deficiency and internal heat was established by feeding with a high-sugar and high-fat diet and injection of thyroxine and streptozotocin. The successfully modeled rats were randomized into model control， Yunvjian without Achyranthis Bidentatae Radix （11.8 g·kg-1）， Yunvjian with Achyranthis Bidentatae Radix （12.8 g·kg-1）， and Achyranthis Bidentatae Radix （1.0 g·kg-1） groups （n=10）， and another 10 rats were taken as the normal control group. Each group was administrated with corresponding drugs or saline by gavage for 28 days. The fasting blood glucose （FBG）， fasting insulin （FINS）， total cholesterol （TC）， triglycerides （TG）， low-density lipoprotein cholesterol （LDL-C）， and high-density lipoprotein cholesterol （HDL-C） in rats were measured. Enzyme-linked immunosorbent assay was employed to determine the levels of cyclic adenosine monophosphate （cAMP）， cyclic guanosine monophosphate （cGMP）， triiodothyronine （T3）， thyroxine （T4）， interleukin （IL）-1β， IL-6， tumor necrosis factor （TNF）-α， and C-reactive protein （CRP） in the serum. The histopathological changes of the liver were observed. The expression of lipoxygenase-2 （COX-2） was detected by immunofluorescence. The mRNA levels of nuclear transcription factors-κB （NF-κB）， monocyte chemotactic protein-1 （MCP-1）， and intercellular adhesion molecule-1 （ICAM-1） were determined by real-time polymerase chain reaction （Real-time PCR）.Western blot was employed to determine the protein levels of NF-κB in hibitory protein（IκB） kinase β （IKKβ）， IκBα， and phosphorylated IκBα （p-IκBα） in the liver and the protein levels of NF-κB in the cytoplasm and nucleus. ResultCompared with the normal group， the model group showed elevated levels of FBG， FINS， insulin resistance index， TC， TG， LDL-C， cAMP， T3， T4， IL-1β， IL-6， TNF-α， and CRP， up-regulated mRNA levels of NF-κB， MCP-1， and ICAM-1， and up-regulated protein levels of COX-2， p-IκBα， and nuclear NF-κB （P<0.01）. Compared with the model group， Yunvjian without Achyranthis Bidentatae Radix lowered the levels of FBG， FINS， insulin resistance index， TC， TG， LDL-C， cAMP， T3， T4， IL-1β， IL-6， TNF-α， and CRP， down-regulated the mRNA levels of NF-κB， MCP-1， and ICAM-1， and down-regulated the protein levels of COX-2， p-IκBα and nuclear NF-κB （P<0.05， P<0.01）. Compared with the Yunvjian without Achyranthis Bidentatae Radix， Yunvjian with Achyranthis Bidentatae Radix showed lowered levels of FBG， FINS， insulin resistance index， and inflammatory cytokines， down-regulated mRNA levels of NF-κB， MCP-1， and ICAM-1， and down-regulated protein levels of p-IκBα and nuclear NF-κB （P<0.05， P<0.01）. ConclusionAchyranthis Bidentatae Radix can enhance the performance of Yunvjian in reducing blood glucose and inhibiting inflammation in diabetic rats with the syndrome of yin deficiency and internal heat by down-regulating the IKK/IκB/NF-κB signaling pathway.

Relationship between preschooler's primary tooth caries and parental knowledge-behavior-knowledge of oral health in Hudong community in Pudong new area / 中华全科医师杂志

Objective To explore the primary teeth caries status of preschoolers in Pudong community and its relevant factors of parental knowledge-belief-practice (KBP).Methods Using stratified random cluster sampling,a total of 1 179 children from 9 kindergartens in Hudong community in Pudong new area were examined.Oral cavity check-ups and parental questionnaire survey were conduced to understand the primary teeth caries rate of preschoolers and its related influencing factors.Results The prevalence rate of primary teeth caries was 53.2％ and DMFT 2.79.The prevalence rate of primary teeth caries had statistical significance in different age groups (P ＜ 0.05).Multi-factor analysis showed that the influencing factors of primary teeth caries were age (P ＜ 0.05),parental education level (P ＜ 0.05),parental assistance of teeth brushing (P ＜ 0.05),children eating sweets before bedtime (P ＜ 0.05) and parental awareness for treating primary teeth (P ＜ 0.05).Conclusions The prevalence rate of primary teeth caries is high among the preschoolers of Pudong.And age,parental education level,parental preventive knowledge of teeth caries and poor eating habits are significant factors correlated with the incidence of dental caries in children.

Determination of rehmannioside D in root ofRehmannia glutinosa by HPLC / 中草药

Object To establish an HPLC method for the determination of rehmannioside D in the root of Rehmanniae glutinosa (Gaert.) Libosch. ex Fisch et Mey and provide a limitation for the lowest content of rehmannioside D Methods Chromatographic conditions: Hypersil C 18 (200 mm?4.6 mm, 5 ?m) column, mobile phase: acetonitrile water (4∶96), flow rate: 1 mL/min, room temperature, detection wavelength: 205 nm Results The recovery was 100.6%, and RSD was 2.1% for rehmannioside D. From eight different regions 16 samples of R glutinosa were quantitatively analysed and the contents of rehmannioside D in R glutinosa were 0.137%-0.691% Conclusion The method is rapid, simple and accurate, and can be used for the quality control of R glutinosa The proposed content of rehmannioside D must be no less than 0.15%.

Effect of rehmaionoside A on cyclophosphamide induced leukopenia in mice / 中草药

Object To observe the effects of rehmaionoside A on mice leukopenia models induced by cyclophosphamide. Methods Mice leukopenia models were prepared by ip cyclophosphamide and then treated with rehmaionoside A. The levels of body weight, peripheral hemogram, reticulocyte count, spleen index, bone marrow karyocyte count and contents of bone marrow DNA were determined. Results Rehmaionoside A could obviously raise the counts of white blood cell, red blood cell, platelet, reticulocyte and bone marrow karyocyte, contents of bone marrow DNA, and body weight of the leukopenia model. Conclusion Rehmaionoside A has apparent leukogenic and antianemic actions.